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E9884

Ethylenediaminetetraacetic acid

Name: Ethylenediaminetetraacetic acid
Brand: SIAL

ACS reagent, 99.4-100.6%, powder

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

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About This Item

Linear Formula:

(HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂

CAS Number:

60-00-4

Molecular Weight:

292.24

NACRES:

NA.77

PubChem Substance ID:

24894705

eCl@ss:

39030907

UNSPSC Code:

12352106

EC Number:

200-449-4

MDL number:

MFCD00003541

Beilstein/REAXYS Number:

1716295

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Properties

Quality Level

200

grade

ACS reagent

agency

suitable for SM 4500 - NH3

assay

99.4-100.6%

form

powder

reaction suitability

reagent type: chelator

impurities

≤0.005% insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

ign. residue

≤0.2%

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

water: soluble 0.4 g/L at 20 °C

cation traces

Ca: ≤0.001%, Fe: ≤0.005%, Mg: ≤5 ppm, heavy metals: ≤0.001% (byICP-OES)

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.

Application

Ethylenediaminetetraacetic acid has been used:

as a component of labeling buffer, which is used in the washing step during cell fractionation{155)
for investigating the effect of papain enzyme on wound debridement and other skin issues
for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany. It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.

Other Notes

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Warning

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

target_organs

Respiratory Tract

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Irrit. 2 - STOT RE 2 Inhalation

wgk

WGK 3

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Global Trade Item Number

SKU	GTIN
E9884-100G	04061835544691
E9884-500G	04061835544714
E9884-1KG	04061835544707