S9430
SYBR® Green I nucleic acid gel stain
10,000 × in DMSO
Synonym(s):
DNA stain, SYBR® green gel dye, safer gel stain
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About This Item
form
solution
Quality Level
usage
mL sufficient for 100 mini-gels
greener alternative product characteristics
Designing Safer Chemicals
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sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
General description
SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.
Application
SYBR® Green I nucleic acid gel stain has been used for:
- the quantification of dsDNA
- to stain DNA in polymerase chain reaction (PCR)
- for comet assay technique
- to assess spermatozoon membrane integrity
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
- as a fluorescent dye in flow cytometry
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
- It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Preparation Note
Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.
Legal Information
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
201.2 °F - closed cup
flash_point_c
94 °C - closed cup
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Protocols
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SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
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Articles
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
I Ortiz et al.
Animal reproduction science, 187, 74-78 (2017-10-19)
The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis from neutered dogs were minced and
P A Morin et al.
BioTechniques, 19(2), 223-228 (1995-08-01)
A method for resolving and visualizing genotypes for simple sequence repeat loci on short (20 cm) polyacrylamide gels is described. This method makes use of a modified vertical electrophoresis cell to allow rapid electrophoresis without variation in migration rates due
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S9430-.5ML | 04061837899140 |
| S9430-1ML | 04061837899164 |