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A8592

ANTI-FLAG® M2-Peroxidase (HRP) antibody, Mouse monoclonal

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
peroxidase conjugate
Clone:
M2, monoclonal
Application:
ELISA (i)
Citations:
1073
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biological source

mouse

Quality Level

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GPX1(2876)

General description

The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.

Application

For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.
Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000

Learn more product details in our FLAG® application portal.

Physical form

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Preparation Note

Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料
常规特殊物品

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Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCH6307
SLCF0816
SLCB9703
SLBV3799
SLBT5667

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Articles

聚糖标记

蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时，它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因，它们在细胞表面的表现通常被称为“糖”。

Glycan Labeling

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

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Global Trade Item Number

SKUGTIN
A8592-5X1MG04061833396889
A8592-.2MG04061835511761
A8592-1MG04061833392409