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B4500

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Synonym(s):

BAEE

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About This Item

Empirical Formula (Hill Notation):
C15H22N4O3 · HCl
CAS Number:
2645-08-1
Molecular Weight:
342.82
NACRES:
NA.32
PubChem Substance ID:
24891777
UNSPSC Code:
12352204
EC Number:
220-157-0
MDL number:
MFCD00012579
Beilstein/REAXYS Number:
3781694
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Product Name

Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

Quality Level

200

assay

≥97% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.
The prototype substrate for trypsin.

Application

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts
  • in trypsin activity assay of the tail tendon fascicles from rat

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH1622
0000135115
0000118817
0000118818
0000115477

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Protocols

胰蛋白酶（EC 3.4.21.4）的酶学测定方案

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

Articles

半胱氨酸蛋白酶——木瓜蛋白酶的特性和产品

木瓜蛋白酶是肽酶C1家族的一种半胱氨酸蛋白酶。它由单一多肽链组成，含有三个二硫键和一个酶活性所必需的巯基。

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Kinetic and Structural Differentiation of Trypsin from Different Origins
Aithal M, et al.
BioPharm International, 30(1), 38-+-38-+ (2017)
Maria T Dulay et al.
Analytical chemistry, 77(14), 4604-4610 (2005-07-15)
Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is
The mechanical, structural, and compositional changes of tendon exposed to elastase
Grant TM, et al.
Annals of Biomedical Engineering, 43(10), 2477-2486 (2015)
View All Related Papers

Global Trade Item Number

SKUGTIN
B4500-10G04061833429686
B4500-25G04061833429709