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B7632

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride

Name: N-Benzoyl-Phe-Val-Arg-<I>p</I>-nitroanilide hydrochloride
Brand: SIGMA

chromogenic, protease substrate, ≥98% (TLC), powder

Synonym(s):

N-Benzoyl-L-phenylalanyl-L-valyl-L-arginine-4-nitroanilide hydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₃₃H₄₀N₈O₆ · HCl

CAS Number:

54799-93-8

Molecular Weight:

681.18

NACRES:

NA.32

PubChem Substance ID:

24892011

UNSPSC Code:

12352204

MDL number:

MFCD00038397

Beilstein/REAXYS Number:

3027332

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Properties

Product Name

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

InChI key

PYVSMZDQQUZGPF-JAQKLANPSA-N

Description

General description

A substrate for trypsin, thrombin and reptilase.

N-Benzoyl-Phe-Val-Arg-p-nitroanilide is a chromogenic protease substrate.

Application

-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride has been used: as a substrate: for trypsin-like enzyme in the soluble and particulate fractions of the hyphae
for the thrombin, recombinant and native batroxobin from snake venom
for fibrinolytic enzyme aprE2 in amidolytic activity assay

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

牛血浆凝血酶

凝血酶是一种内溶性丝氨酸蛋白酶，可选择性裂解纤维蛋白原的精氨酸-甘氨酸键，从而形成纤维蛋白并释放纤维蛋白肽A和B。

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Synthesis of Bz-Phe(p-No2)-Val-Arg-pNA-and its specificity to thrombin-like enzymes: similarity of a commercial product S-2160 to the nitrated compound.

K Takada et al.

Thrombosis research, 20(5-6), 533-572 (1980-12-01)

Heparin and protease inhibition. II. The role of heparin in the ATIII inactivation of thrombin, plasmin, and trypsin.

G F Smith et al.

Thrombosis research, 22(1-2), 115-133 (1981-04-01)

Methodological considerations on antithrombin determination.

U Abildgaard

Acta chirurgica Scandinavica. Supplementum, 509, 97-99 (1982-01-01)

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Global Trade Item Number

SKU	GTIN
B7632-50MG	04061832912806
B7632-10MG	04061833442005
B7632-5MG	04061833442012