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C2978

CelLytic™ M

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

Synonym(s):

Whole-cell protein extraction solution

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About This Item

NACRES:
NA.56
UNSPSC Code:
41116134
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Quality Level

200

form

solution

storage temp.

room temp

General description

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Features and Benefits

  • Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
  • Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
  • Convenient: Ready-to-use reagent requires no scraping from culture plates
  • Fast: Rapid cell lysis at room temperature.

Preparation Note

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).

Analysis Note

Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
099M4091V
099M4049V
11190504
039M4063V
069M4062V

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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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Global Trade Item Number

SKUGTIN
C2978-250ML04061838445568
C2978-40ML04061838248190
C2978-10ML04061838248183
C2978-50ML04061838445575
C2978-60ML04061838112828