CE0050
CelLytic™ MEM Protein Extraction Kit
Synonym(s):
Membrane protein extraction kit
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About This Item
NACRES:
NA.56
UNSPSC Code:
41116134
General description
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.
Application
CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.
Sufficient reagents supplied for 80 tests.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 2
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2
Regulatory Information
常规特殊物品
低风险生物材料
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Elisabeth P Carpenter et al.
Current opinion in structural biology, 18(5), 581-586 (2008-08-05)
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to
Yue Zhao et al.
Cancer medicine, 7(8), 3977-3987 (2018-07-06)
Esophageal squamous cell carcinoma (ESCC) is a malignant disease with poor prognosis. Because of early metastasis prior to diagnosis and therapeutic resistance, ESCC has become one of the leading causes of cancer-related death. Here, we investigated the clinicopathological significance of
Wei He et al.
Annals of translational medicine, 10(4), 218-218 (2022-03-15)
Colonic mucosal injuries are an important manifestation of ulcerative colitis (UC), which is related to hypoxia-induced glycolysis in colonic mucosal epithelial cells (cmECs). Panax notoginseng (PN) promotes the repair of colonic mucosal injuries by inhibiting hypoxia-induced glycolysis in cmECs; However
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CE0050-1KT | 04061835518050 |