Isolated Mitochondria Staining Kit

Name: Isolated Mitochondria Staining Kit
Brand: SIGMA

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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About This Item

UNSPSC Code:

12352207

EC Number:

217-896-6

NACRES:

NA.32

Technique(s):

flow cytometry: suitable, protein staining: suitable

Usage:

kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)

Storage temp.:

−20°C

Detection method:

fluorometric

Shipped in:

dry ice

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Properties

Quality Level

200

usage

kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitable, protein staining: suitable

fluorescence

λ_ex 490 nm; λ_em 590 nm (JC-1 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C

Description

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Biochem/physiol Actions

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Features and Benefits

Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation