CY0100
Cytochrome c Reductase (NADPH) Assay Kit
1 kit sufficient for 100 tests, determining cytochrome c reductase activity
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Biochem/physiol Actions
Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.
Features and Benefits
- Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation
- Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics
- Analyze enrichment and function of ER
Analysis Note
The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.
Kit Components Only
Product No.
Description
- Assay Buffer 100 mL
- Enzyme Dilution Buffer 25 mL
- Cytochrome c 50 mg
- Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
- Cytochrome c Oxidase Inhibitor Solution 1 mL
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg
signalword
Danger
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
target_organs
Eyes,Central nervous system
wgk
WGK 3
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - STOT SE 2
Regulatory Information
监管及禁止进口产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Related Content
D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by
Amanda Spring de Almeida et al.
Life sciences, 276, 119469-119469 (2021-04-04)
Breast cancer-induced chronic pain is usually treated with opioids, but these compounds cause various adverse effects. Transient receptor potential ankyrin 1 (TRPA1) is involved in cancer pain; also, endogenous TRPA1 agonists are associated with cancer pain development. The aim of
C O Lemley et al.
The Journal of endocrinology, 205(3), 233-241 (2010-03-13)
Elevated rates of steroid clearance may lead to lower reproductive success in several mammalian species. Cytochrome P450 (EC 1.14.14.1) and aldo-keto reductases (AKR; EC 1.1.1.145-151) are involved in the first phase of steroid inactivation, before second phase conjugation and excretion
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CY0100-1KT | 04061826754184 |