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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Usage:
sufficient for 100 tests
Storage temp.:
−20°C
Shipped in:
dry ice
Quality Level
usage
sufficient for 100 tests
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... COX1(4512)
Application
The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.
Biochem/physiol Actions
Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
Features and Benefits
- Simple, optimized protocol - Obtain reproducible results without special training needs
- Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
- Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
- May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
- Use to analyze the intactness of mitochondrial membranes
- Simple colorimetric measurement of solution
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
动植物源性产品
低风险生物材料
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Articles
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| CYTOCOX1-1KT | 04061833553237 |
