Skip to Content
Merck
CN

Key Documents

View All Documentation

D0428

1 kb DNA Ladder

for DNA electrophoresis

Synonym(s):

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.25
UNSPSC Code:
41105335
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

100, 200

form

liquid

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.

Other Notes

Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
For optimal resolution, the recommended agarose gel concentration is 0.75%.

Legal Information

SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCK0031
SLCJ3543
SLCF6197
SLCB4316
SLBT9675

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Related Content

mRNA 合成

用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

GenElute Five-Minute Plasmid Miniprep Kit: The Fastest Method for Plasmid Preparation
View All Related Content
W S Choe et al.
Biotechnology progress, 17(6), 1107-1113 (2001-12-12)
The direct chemical extraction of recombinant L1 protein (the major capsid protein of human papillomavirus type 16) from the cytoplasm of E. coli HMS174(DE3) has recently been demonstrated at high cell density (to OD(600) = 160) without the use of
Yu Zhou et al.
Journal of experimental botany, 59(10), 2803-2813 (2008-06-03)
The turnip crinkle virus-based vector TCV-GFP Delta CP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing. TCV-GFP Delta CP, which had been constructed by replacing the coat protein (CP) gene with a green fluorescent
Luca Cocolin et al.
Applied and environmental microbiology, 70(3), 1347-1355 (2004-03-10)
In this paper we describe the development of a PCR protocol to specifically detect Brettanomyces bruxellensis and B. anomalus. Primers DB90F and DB394R, targeting the D1-D2 loop of the 26S rRNA gene, were able to produce amplicons only when the
View All Related Papers

Global Trade Item Number

SKUGTIN
D0428-1VL04061835572458