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Merck
CN

D4522

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonym(s):

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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About This Item

CAS Number:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
Biological source:
bovine pancreas
Form:
lyophilized powder
Solubility:
water: slightly soluble
Storage temp.:
−20°C
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biological source

bovine pancreas

Quality Level

grade

Molecular Biology

type

Type XV

form

lyophilized powder

solubility

water: slightly soluble

storage temp.

−20°C

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
One mg DNA is approx. 20 A260 units.
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

低风险生物材料

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Claudia Gomes et al.
Life science alliance, 4(11) (2021-09-11)
High levels of autoimmune antibodies are observed in COVID-19 patients but their specific contribution to disease severity and clinical manifestations remains poorly understood. We performed a retrospective study of 115 COVID-19 hospitalized patients with different degrees of severity to analyze
Anastasia S Singatulina et al.
Cell reports, 27(6), 1809-1821 (2019-05-09)
PARP-1 synthesizes long poly(ADP-ribose) chains (PAR) at DNA damage sites to recruit DNA repair factors. Among proteins relocated on damaged DNA, the RNA-binding protein FUS is one of the most abundant, raising the issue about its involvement in DNA repair.
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)



Global Trade Item Number

SKUGTIN
D4522-1MG04061833565384
D4522-5MG04061833565391
D4522-5X5MG04061833565407