D9163
DL-Dithiothreitol
suitable for electrophoresis, ≥99%
Synonym(s):
threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
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About This Item
Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
Molecular Weight:
154.25
UNSPSC Code:
12161504
NACRES:
NB.22
PubChem Substance ID:
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:
Quality Level
assay
≥99%
form
powder
reaction suitability
reagent type: reductant
technique(s)
electrophoresis: suitable
mp
40-44 °C, 41-44 °C (lit.)
solubility
H2O: 50 mg/mL, clear, colorless to very faintly yellow
cation traces
heavy metals (as Pb): ≤10 ppm
absorption
≤0.04 at 280 at 0.02 M
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Analysis Note
Tested for use in reduced SDS-PAGE
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D9163-25G | 04061835521814 |
| D9163-1G | 04061833590386 |
| D9163-5G | 04061833590393 |