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About This Item
form
resin (dry)
crosslinking
cross-linked
mol wt
protein Mr 800-5000 Da (fractionation range), protein Mr 8000 Da (exclusion limit)
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0033-01
storage condition
dry media
technique(s)
liquid chromatography (LC): suitable
matrix
cross-linked dextran
particle size
50-150 μm (dry), 85-255 μm (wet)
cleaning in place
2-13
operating pH
2-13
separation technique
size exclusion (SEC)
storage temp.
2-30°C
General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).
Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Excellent recovery and minimum sample dilution
- Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
- BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications
Preparation Note
Analysis Note
Legal Information
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Storage Class
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Protocols
如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。
本页介绍如何使用Cytiva的sephacryl色谱柱进行分离，该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Articles
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Related Content
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-0033-01 | 04061834083344 |