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GE17-0043-01

Sephadex^® G-50

Name: Sephadex<SUP>®</SUP> G-50
Brand: SIGMA

Medium, Cytiva, pack of 100 g

Synonym(s):

SEC resin, buffer exchange resin, chromatography resin, cross-linked dextran, cytiva 17-0043-01, desalting matrix, filtration gel, size-exclusion resin

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About This Item

CAS Number:

9004-54-0

UNSPSC Code:

23151817

NACRES:

NA.56

MDL number:

MFCD23701222.obs1210407

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Properties

form

resin

crosslinking

cross-linked

mol wt

protein M_r 3-20 kDa (fractionation range), protein M_r 30 kDa (exclusion limit), M_p 0.5-10 kDa (dextrans fractionation range)

feature

autoclavable In wet form (pH 7.0) at 120°C for 30 min

packaging

pack of 100 g

manufacturer/tradename

Cytiva

technique(s)

liquid chromatography (LC): suitable

matrix

cross-linked dextran

matrix active group

dextran

swelling

1 g swells to 14-15 mL

particle size

103-320 μm (wet), 50-150 μm (dry)

cleaning in place

2-13

working range

2-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

SMILES string

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

Description

General description

Sephadex^® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

Application

Sephadex^® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex^® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex^® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex^® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and Low operating pressures are required.

Features and Benefits

Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
Suitable for purification of DNA from small molecules by gel filtration
Larger bead size for large scale group separations where high flow rates and Low operating pressures are required.
Classic gel filtration medium.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

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Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c