packaging
pack of 1 L
manufacturer/tradename
Cytiva 17-0120-01
matrix
4% agarose
particle size
45-165 μm
cleaning in place
4-9
working range
4-9
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
General description
Sepharose™ 4B is a well-proven Agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
Application
Sepharose™ is a bead-formed Agarose-based gel filtration matrix. Sepharose™ is available with 3 different Agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents
Features and Benefits
- 4% Agarose gel filtration media.
- Proven base matrix for coupling affinity ligands
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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The agarose migration inhibition technique for in vitro demonstration of cell-mediated immunity in man. A review.
J E Clausen
Danish medical bulletin, 22(5), 181-194 (1975-07-01)
Jana Wolf et al.
The EMBO journal, 33(14), 1514-1526 (2014-05-30)
The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds
Sudhir Chandna et al.
International journal of radiation biology, 90(5), 401-406 (2014-02-18)
Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. Macrocolony formation
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-0120-01 | 04061837620010 |