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GE17-0851-01

Disposable PD 10 Desalting Columns

Cytiva 17-0851-01, pack of 30 pieces

Synonym(s):

Disposable desalting columns, PD-10 columns

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816
Technical Service
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form

prepacked column

crosslinking

cross-linked

feature

wetted part: no

packaging

pack of 30 pieces

manufacturer/tradename

Cytiva 17-0851-01

storage condition

(0.15% kathon)

parameter

2.5 mL sample volume

technique(s)

liquid chromatography (LC): suitable

bed size

14.5 mm × 50 mm

bed volume

8.3 mL

column I.D.

15 mm

matrix

cross-linked dextran

matrix active group

dextran

particle size

50-150 μm

cleaning

2-13

working range

2-13

suitability

suitable for bioprocess medium

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

General description

Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.

The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.

Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.

Features and Benefits

  • Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex® G-25 Medium.
  • Flexible protocols allow runs by gravity flow or centrifugation.
  • Easy to use and equilibrate; apply sample; elute.
  • Preparation of the samples in the range of 1.0 to 2.5 mL by gravity flow and 1.75 to 2.5 mL with centrifugation.
  • Desalt multiple samples in parallel with a desalting capacity >90%.
  • Recovery in the range 70 to >95%.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Legal Information

Sephadex is a registered trademark of Cytiva


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Storage Class

12 - Non Combustible Liquids

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Not applicable

flash_point_c

Not applicable

Regulatory Information

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Protocols

如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

View All Protocols

Articles

实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

本页介绍了高效的色谱柱填充和抗体亲和层析的制备。

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

View All Articles

Ye Guan et al.
eLife, 7 (2018-04-06)
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell



Global Trade Item Number

SKUGTIN
GE17-0851-0104061835573127