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About This Item
form
prepacked column
crosslinking
cross-linked
feature
wetted part: no
packaging
pack of 30 pieces
manufacturer/tradename
Cytiva 17-0851-01
storage condition
(0.15% kathon)
parameter
2.5 mL sample volume
technique(s)
liquid chromatography (LC): suitable
bed size
14.5 mm × 50 mm
bed volume
8.3 mL
column I.D.
15 mm
matrix
cross-linked dextran
matrix active group
dextran
particle size
50-150 μm
cleaning
2-13
working range
2-13
suitability
suitable for bioprocess medium
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.
Features and Benefits
- Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex® G-25 Medium.
- Flexible protocols allow runs by gravity flow or centrifugation.
- Easy to use and equilibrate; apply sample; elute.
- Preparation of the samples in the range of 1.0 to 2.5 mL by gravity flow and 1.75 to 2.5 mL with centrifugation.
- Desalt multiple samples in parallel with a desalting capacity >90%.
- Recovery in the range 70 to >95%.
Preparation Note
Legal Information
signalword
Warning
hcodes
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Protocols
如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
Articles
实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
本页介绍了高效的色谱柱填充和抗体亲和层析的制备。
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-0851-01 | 04061835573127 |
