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H8651

HEPES sodium salt

Name: HEPES sodium salt
Brand: SIGMA

BioXtra, ≥99.0% (titration)

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):

C₈H₁₇N₂NaO₄S

CAS Number:

75277-39-3

Molecular Weight:

260.29

NACRES:

NA.25

PubChem Substance ID:

24895527

UNSPSC Code:

12161700

EC Number:

278-169-7

MDL number:

MFCD00036463

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Properties

product line

BioXtra

assay

≥99.0% (titration)

form

powder

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

pH

10.0-12.0 (1 M in H₂O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H₂O: 1 M, clear, colorless

anion traces

chloride (Cl^-): ≤0.05%, sulfate (SO₄^2-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Pb: ≤0.001%, Zn: ≤0.0005%

absorption

≤0.2 at 260 in H₂O at 1 M, ≤0.2 at 280 in H₂O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Other Notes

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
H8651-500G	04061833811092
H8651-100G	04061833810934
H8651-25G	04061833810989