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H9882

4-Hydroxybenzhydrazide

Name: 4-Hydroxybenzhydrazide
Brand: SIGMA

powder, ≥97%

Synonym(s):

4-Hydroxybenzoic hydrazide, 4-Hydroxybenzoylhydrazine, p-Hydroxybenzoic acid hydrazide

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About This Item

Linear Formula:

HOC₆H₄CONHNH₂

CAS Number:

5351-23-5

Molecular Weight:

152.15

NACRES:

NA.32

PubChem Substance ID:

24278478

UNSPSC Code:

12352204

EC Number:

226-326-5

MDL number:

MFCD00007605

Beilstein/REAXYS Number:

473052

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Properties

Product Name

4-Hydroxybenzhydrazide, ≥97%

biological source

synthetic

Quality Level

100

assay

≥97%

form

powder

mp

264-266 °C (dec.) (lit.)

solubility

acetic acid: water (1:1): 25 mg/mL, clear, colorless to faintly yellow

fluorescence

λ_ex 360 nm; λ_em 425 nm (reacted with 5-hydroxymethyl-furaldehydezinc acetate)

SMILES string

NNC(=O)c1ccc(O)cc1

InChI

1S/C7H8N2O2/c8-9-7(11)5-1-3-6(10)4-2-5/h1-4,10H,8H2,(H,9,11)

InChI key

ZMZGIVVRBMFZSG-UHFFFAOYSA-N

Description

Application

4-Hydroxybenzhydrazide has been used:

for determining the concentration of maltose from starch digestion
for analysis of sugars from fructan containing food hydrolysate
in colorimetric assay for reducing sugar estimation from processed feedstuff

Biochem/physiol Actions

4-Hydroxybenzhydrazide (PAHBAH), is used for assaying α-amylase and glucoamylase activities. The assay based on PAHBAH measures non-specifically the free reducing sugar groups present in samples at 415nm colorimetrically.

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pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

转化酶的酶学测定

在测定转化酶活性时，使用了一种在410nm处的分光光度终点反应法进行测定。在55 ºC、pH 4.5条件下，一单位的转化酶每分钟可将1.0 μmole的蔗糖水解为转化糖。

Enzymatic Assay of Invertase

Extrusion induced low-order starch matrices: Enzymic hydrolysis and structure

Zhang B, et al.

Carbohydrate Polymers, 134(10), 485-496 (2015)

Measurement of total fructan in foods by enzymatic/spectrophotometric method: collaborative study

McCleary BV, et al.

Journal of AOAC (Association of Official Analytical Chemists) International, 83(2), 356-364 (2000)

Efficacy of reducing sugar and phenol-sulfuric acid assays for analysis of soluble carbohydrates in feedstuffs

Hall, Mary Beth

Anim. Feed Sci. Technol., 185(1-2), 94-100 (2013)

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Global Trade Item Number

SKU	GTIN
H9882-100G	04061835557226
H9882-25G	04061835521883