L1412
Lysing Enzymes from Trichoderma harzianum
lyophilized powder
Synonym(s):
Glucanex®
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About This Item
UNSPSC Code:
12352204
Specific activity:
≥10 units/g
form
lyophilized powder
specific activity
≥10 units/g
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Application
Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
- for protoplast isolation
- for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation
- for the lysis of Aspergillus niger vegetative cells
Biochem/physiol Actions
Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.
Analysis Note
Contains β-glucanase, cellulase, protease, and chitinase activities
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).
Legal Information
A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Katia Cristina Dantas et al.
PloS one, 13(1), e0190408-e0190408 (2018-01-18)
Although early and rapid detection of histoplasmosis is essential to prevent morbidity and mortality, few diagnostic tools are available in resource-limited areas, especially where it is endemic and HIV/AIDS is also epidemic. Thus, we compared conventional and molecular methods to
Elena Wiederhold et al.
Molecular & cellular proteomics : MCP, 8(2), 380-392 (2008-11-13)
Transport of solutes between the cytosol and the vacuolar lumen is of crucial importance for various functions of vacuoles, including ion homeostasis; detoxification; storage of different molecules such as amino acids, phosphate, and calcium ions; and proteolysis. To identify proteins
Xiao-Lian Yuan et al.
Molecular genetics and genomics : MGG, 279(1), 11-26 (2007-10-06)
The expression of inulinolytic genes in Aspergillus niger is co-regulated and induced by inulin and sucrose. We have identified a positive acting transcription factor InuR, which is required for the induced expression of inulinolytic genes. InuR is a member of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L1412-10G | 04061835557448 |
| L1412-25G | 04061835518180 |
| L1412-5G | 04061833949412 |