Key Documents

View All Documentation

M4765

N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide

Name: N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide
Brand: SIGMA

elastase substrate, chromogenic, ≥98% (HPLC), powder

Synonym(s):

N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide, N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₇H₃₈N₆O₉

CAS Number:

70967-90-7

Molecular Weight:

590.63

NACRES:

NA.32

PubChem Substance ID:

24896943

UNSPSC Code:

12352204

MDL number:

MFCD00077137

Beilstein/REAXYS Number:

6266359

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide, elastase substrate

Quality Level

300

assay

≥98% (HPLC)

form

powder

solubility

DMF: soluble 25 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

[H][C@]1(CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(=O)OC)C(=O)N[C@@H](C(C)C)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C27H38N6O9/c1-15(2)23(26(38)30-18-8-10-19(11-9-18)33(40)41)31-25(37)20-7-6-14-32(20)27(39)17(4)29-24(36)16(3)28-21(34)12-13-22(35)42-5/h8-11,15-17,20,23H,6-7,12-14H2,1-5H3,(H,28,34)(H,29,36)(H,30,38)(H,31,37)/t16-,17-,20-,23-/m0/s1

InChI key

VLVGCNNWNUERRZ-OSAZLGQLSA-N

Description

General description

Substrate for human leukocyte elastase.

Application

MeO-SucAAPVpNA has been used to measure neutrophil elastase activity:

in response to zinc supplementation using human and mice neutrophils in vitro
to determine the functional properties of aged neutrophils to assess their role in supporting metastasis
to determine the anti-elastase action of Sclerocarya birrea (A. Rich.) Hochst

Biochem/physiol Actions

Human leukocyte elastase (HLE) is a serine proteinase associated with inflammation and tissue degradation. It is one of the most destructive proteases and its role in the development of pulmonary emphysema has been investigated. This peptide is selectively hydrolyzed by HLE, and other elastase of different species and cells. Cleavage of MeO-SucAPVVpNA by an elastase-like protease is critical for the synthesis of platelet-activating factor (PAF) induced by tumor necrosis factor-α (TNF-α) and interleukin-1α (IL-1α). The rate of enzymatic hydrolysis of the substrate is followed by the increase in absorbance due to the release of 4-nitroaniline. The absorbance is routinely measured at 400 nm or higher.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Elastase Function and Importance

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Global Trade Item Number

SKU	GTIN
M4765-1G	04061823207027
M4765-10MG	04061834059165
M4765-250MG	04061832917849
M4765-50MG	04061834059172