Lactate Dehydrogenase Assay Kit

Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage.

The kit is suitable for lactate dehydrogenase activity determination in serum, plasma, and other sources. It is also suitable for the characterization and quality control of LDH production, as well as for the screening and evaluation of LDH modulators in drug discovery.

The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.