N2752
4-Nitrophenyl palmitate
lipase substrate, chromogenic, ≥98% (TLC), powder
Synonym(s):
p-Nitrophenyl palmitate, Hexadecanoic acid 4-nitrophenyl ester
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About This Item
Empirical Formula (Hill Notation):
C22H35NO4
CAS Number:
Molecular Weight:
377.52
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
216-084-9
MDL number:
Beilstein/REAXYS Number:
1891754
Product Name
4-Nitrophenyl palmitate, lipase substrate
Quality Level
assay
≥98% (TLC)
form
powder
solubility
chloroform: 100 mg/mL, clear, colorless to faintly yellow
storage temp.
−20°C
SMILES string
CCCCCCCCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3
InChI key
LVZSQWIWCANHPF-UHFFFAOYSA-N
General description
4-Nitrophenyl palmitate is a substrate for lipase enzyme activity. Lipase hydrolyzes 4-nitrophenyl palmitate and yields the yellow colored product 4-nitrophenol, which is measurable spectrophotometrically at 410 nm. This method is advantageous due to its short reaction time and facile spectrophotometric analyses. The cell-bound lipase has preference for 4-nitrophenyl palmitate as substrate than the extracellular lipase.
Application
4-nitrophenyl palmitate has been used as a substrate for lipase enzyme activity.
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Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application
Zarinviarsagh M, et al.
Lipids in Health and Disease, 16(1), 177-177 (2017)
Seon-Woo Lee et al.
Applied microbiology and biotechnology, 65(6), 720-726 (2004-09-15)
The construction and screening of metagenomic libraries constitute a valuable resource for obtaining novel biocatalysts. In this work, we present the construction of a metagenomic library in Escherichia coli using fosmid and microbial DNA directly isolated from forest topsoil and
Kai Yuan et al.
Journal of industrial microbiology & biotechnology, 46(8), 1091-1101 (2019-06-21)
Metabolic fluxes during lipase production by Bacillus subtilis CICC 20034 in synthetic medium were studied using metabolic flux analysis (MFA). The MFA showed that lipase production was dependent on, and coupled to the tributyrin uptake rate, formation of biomass, lactate
Global Trade Item Number
| SKU | GTIN |
|---|---|
| N2752-1G | 04061835557981 |
| N2752-5G | 04061835512782 |