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N3910

Neutralization Solution B

Name: Neutralization Solution B
Brand: SIGMA

Synonym(s):

DNA neutralization solution, neutralization buffer DNA extraction

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161703

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Properties

grade

Molecular Biology

Quality Level

200

form

liquid

feature

Direct PCR

technique(s)

DNA amplification: suitable, DNA extraction: suitable, PCR: suitable

input

crude DNA

storage temp.

−20°C

Description

General description

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp^™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp^™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp^™ PCR Reaction Mix.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

pictograms

GHS07,GHS09

signalword

Warning

hcodes

H315,H317,H319,H411

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

Regulatory Information

低风险生物材料

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Protocols

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish

Varshney GK, et al.

Nature Protocols, 11(12), 2357-2357 (2016)

Neurogenesis decreases during brain maturation from adolescence to adulthood.

Jun He et al.

Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)

Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we

Activated K-ras and INK4a/Arf deficiency cooperate during the development of pancreatic cancer by activation of Notch and NF-κB signaling pathways.

Zhiwei Wang et al.

PloS one, 6(6), e20537-e20537 (2011-06-16)

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-related death in the United States, suggesting that novel strategies for the prevention and treatment of PDAC are urgently needed. K-ras mutations are observed in >90% of pancreatic cancer, suggesting

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Global Trade Item Number

SKU	GTIN
N3910-2.5ML	04061837301117
N3910-240ML	04061837301131
N3910-6ML	04061837301186
N3910-1.2ML	04061837301094
N3910-24ML	04061834116387
N3910-60ML	04061837301162