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N8638

4-Nitrophenyl β-D-N,N′,N′′-triacetylchitotriose

Name: 4-Nitrophenyl &#946;-<SC>D</SC>-N,N&#8242;,N&#8242;&#8242;-triacetylchitotriose
Brand: SIGMA

chitinase substrate; lysozyme substrate, ≥98% (TLC), powder

Synonym(s):

4-Nitrophenyl b-D-N,N′,N′′-triacetylchitotriose, 4-Nitrophenyl beta-D-N,N′,N′′-triacetylchitotriose, N-[2-[5-acetamido-6-[5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-3-yl]oxy-4-hydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide

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About This Item

Empirical Formula (Hill Notation):

C₃₀H₄₄N₄O₁₈

CAS Number:

7699-38-9

Molecular Weight:

748.69

UNSPSC Code:

12352204

PubChem Substance ID:

24897686

NACRES:

NA.32

MDL number:

MFCD00057914

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Properties

Product Name

4-Nitrophenyl β-D-N,N′,N′′-triacetylchitotriose, chitinase and lysozyme substrate

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

DMSO: 9.80-10.20 mg/mL, clear to slightly hazy, colorless to yellow

storage temp.

2-8°C

SMILES string

CC(=O)NC1C(O)C(O)C(CO)OC1OC2C(O)C(NC(C)=O)C(OC2CO)OC3C(O)C(NC(C)=O)C(OC3CO)Oc4ccc(cc4)N(=O)=O

InChI

1S/C30H44N4O18/c1-11(38)31-19-23(42)22(41)16(8-35)48-29(19)51-27-18(10-37)50-30(21(25(27)44)33-13(3)40)52-26-17(9-36)49-28(20(24(26)43)32-12(2)39)47-15-6-4-14(5-7-15)34(45)46/h4-7,16-30,35-37,41-44H,8-10H2,1-3H3,(H,31,38)(H,32,39)(H,33,40)

InChI key

UWYYAJFSFHIFNY-UHFFFAOYSA-N

Description

General description

4-Nitrophenyl β-D-N,N′,N′′-triacetylchitotriose is a substrate for hydrolytic activity of chitinase enzyme, with the release of chromogenic end product p-nitrophenol, measured at 405 nm.

Chitinase and lysozyme substrate

Application

4-Nitrophenyl β-D-N,N′,N′′-triacetylchitotriose has been used as a substrate for determining the endochitinase activity of a novel protein isolated from the yam (Dioscorea opposita Thunb.) (DOI), enzyme activity of recombinant chitinase CvChi45.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Osawa, T. and Nakazawa, Y.

Biochimica et Biophysica Acta, 130, 56-56 (1966)

Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli

Lobo MDP, et al.

BMC biotechnology, 13(1), 46-46 (2013)

A Novel, Stable, Estradiol-Stimulating, Osteogenic Yam Protein with Potential for the Treatment of Menopausal Syndrome.

Kam Lok Wong et al.

Scientific reports, 5, 10179-10179 (2015-07-15)

A novel protein, designated as DOI, isolated from the Chinese yam (Dioscorea opposita Thunb.) could be the first protein drug for the treatment of menopausal syndrome and an alternative to hormone replacement therapy (HRT), which is known to have undesirable

Global Trade Item Number

SKU	GTIN
N8638-1MG	04061826676363
N8638-5MG	04061834118589