O4387
Oligo(dT)23, Anchored
70 μM in H2O
Synonym(s):
Oligo(dT)23 Anchored Primer
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106305
Biological source:
synthetic (organic)
Form:
solution
Storage temp.:
−20°C
Solubility:
water: soluble at 20 °C
biological source
synthetic (organic)
Quality Level
form
solution
usage
0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20)
feature
PCR Additives
concentration
70 μM in H2O
technique(s)
cDNA synthesis: suitable
color
colorless
solubility
water: soluble at 20 °C
shipped in
wet ice
storage temp.
−20°C
General description
Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence. The optimal conditions for the concentration of enhanced AMV reverse transcriptase, template RNA, primers, and amplification parameters will depend on the system being utilized and should be determined empirically.
Application
Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).
The Anchored Oligo(dT)23 Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)23 primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.
Features and Benefits
- In the process of preparing cDNA libraries, when there is incomplete or missing sequence information or when specific primers are not suitable, Oligo(dT)23, Anchored primers can be employed together with random nonamers (Product No. R 7647).
- These primers can serve as a substitute for reverse transcription primers in tasks like first-strand cDNA synthesis, cDNA library construction, and various other applications.
- Following transcription, the anchored oligo(dT)23 primers exhibit a reduced ability to initiate priming at higher temperatures (up to 65 °C), ensuring that they do not interfere with PCR.
- Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.
Other Notes
For laboratory use only. Not for drug, household or other uses.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Regulatory Information
常规特殊物品
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Protocols
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
Articles
分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| O4387-.1ML | 04061834233831 |