Anti-β-O-Linked N-Acetylglucosamine antibody, Mouse monoclonal

Monoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH. This antibody recognizes O-GlcNAc in β-O-glycosidic linkage to both serine and threonine and does not cross react with α-linked Ser/Thr-O-GlcNAc, α-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G. O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) has 13.5 tetratricopeptide-repeats (TPR) and a globular catalytic domain. The gene encoding this protein is localized on human chromosome Xq13.1.

synthetic peptide YSPTS(O-GlcNAc)PSK.

Monoclonal Anti-β-O-Linked N-Acetylglucosamine antibody produced in mouse has been used in Western Blotting.

O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) has a role in the addition of O-linked N-acetylglucosamine (O-GlcNAc) to serine/threonine residues in the protein of interest. It is crucial for O-GlcNAcylation of RNA polymerase II. Absence of OGT has been shown to reduce transcription and pol II promoter occupancy. The gene is upregulated in breast cancer tissues, colon cancer, lung cancer and laryngeal cancer. This protein might be associated with tumorigenesis and metastasis.

The antibody recognizes O-GlcNAc in β-O-glycosidic linkage to both serine and threonine and does not cross reacts with α-linked Ser/Thr-O-GlcNAc, α-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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