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P0501

Phenolphthalein β-D-glucuronide

Name: Phenolphthalein β-<SC>D</SC>-glucuronide
Brand: SIGMA

β-glucuronidase substrate

Synonym(s):

Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein glucuronic acid, Phenolphthalein mono-β-D-glucosiduronic acid

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About This Item

Empirical Formula (Hill Notation):

C₂₆H₂₂O₁₀

CAS Number:

15265-26-6

Molecular Weight:

494.45

UNSPSC Code:

12352204

NACRES:

NA.32

PubChem Substance ID:

24898108

MDL number:

MFCD00135881

Beilstein/REAXYS Number:

68526

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Properties

Quality Level

200

assay

≥97% (TLC)

form

powder

solubility

H₂O: soluble 100 mg/mL

storage temp.

−20°C

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5

InChI

1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1

InChI key

FXJYOZKDDSONLX-XADSOVDISA-N

Description

Application

Phenolphthalein β-D-glucuronide has been used as a substrate for the detection of β-D-glucuronidase activity in fecal bacteria.

β-glucuronidase activity was determined by the amount of phenolphthalein released from 0.01 M henolphthalein β-D-glucuronide.

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Articles

Drug Conjugate Analysis using β-Glucuronidases

Drug conjugate analysis and the enzymatic hydrolysis of glucuronides

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Effects of two pomace insoluble fibres on the activities of faecal bacterial enzymes and intestinal health

Chau CF and Chen CH

European Food Research and Technology, 222(5-6), 681-685 (2006)

Improvement in intestinal function of hamsters as influenced by consumption of polysaccharide-rich sage weed extracts

Huang YL and Chau CF

Food Chemistry, 133(4), 1618-1623 (2012)

Intestinal health benefits of the water-soluble carbohydrate concentrate of wild grape (Vitis thunbergii) in hamsters

Huang YL, et al.

Journal of Agricultural and Food Chemistry, 60(19), 4854-4858 (2012)

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Global Trade Item Number

SKU	GTIN
P0501-25MG	04061832918433
P0501-100MG	04061834304081
P0501-500MG	04061834304098