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P8215

Protease Inhibitor Cocktail

Name: Protease Inhibitor Cocktail
Brand: SIGMA

DMSO solution, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use with fungal and yeast extracts, DMSO solution

Synonym(s):

Protease Inhibitor Mix, Protease inhibitor

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.54

EC Number:

200-664-3

MDL number:

MFCD00677817

Form:

DMSO solution

Solubility:

water: soluble

Storage temp.:

−20°C

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Properties

Product Name

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

Quality Level

300

form

DMSO solution

solubility

water: soluble

storage temp.

−20°C

Description

General description

The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.

Application

Protease Inhibitor Cocktail has been used as a component in:

FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
potassium phosphate buffer (pbs) to digest chitin
extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis

Biochem/physiol Actions

Inhibits serine, cysteine, aspartic, and metalloproteases.

Features and Benefits

Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.

Physical form

Solution in DMSO

Preparation Note

One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.

Other Notes

AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.