R3654
Anti-RbAp48 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Retinoblastoma-binding protein
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
2
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 48 kDa
species reactivity
human
technique(s)
indirect immunofluorescence: 1-2 μg/mL using HeLa cells fixed with paraformaldehyde-Triton, western blot: 0.5-1 μg/mL using MCF7 nuclear extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RBBP4(5928)
mouse ... Rbbp4(19646)
rat ... Rbbp4(313048)
General description
Histone binding protein (RbAp48, also known as also known as RBBP4 or NURF55) contains 425 amino acids, tryptophan-aspartic acid (WD)-domain containing protein isolated as a retinoblastoma binding protein (RB1).
Immunogen
synthetic peptide corresponding to amino acids 1-14 of human RbAp48, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat.
Application
Anti-RbAp48 antibody produced in rabbit has been used in immunoprecipitation and western blot analysis. It may also be used in immunofluorescence.
Biochem/physiol Actions
RbAp48 is found in association with chromatin remodeling complexes Sin3A/histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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Related Content
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Nucleic Acids Research, 34(11), 3288-3288 (2006)
Gohei Nishibuchi et al.
The Journal of biological chemistry, 289(42), 28956-28970 (2014-09-06)
Histone H3K4 methylation has been linked to transcriptional activation. KDM5A (also known as RBP2 or JARID1A), a member of the KDM5 protein family, is an H3K4 demethylase, previously implicated in the regulation of transcription and differentiation. Here, we show that
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R3654-200UL | 04061835752546 |