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Merck
CN

T1323

Monoclonal Anti-ε-Tubulin antibody produced in mouse

clone TUB-11, purified immunoglobulin, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
TUB-11, monoclonal
Application:
ARR, ELISA (i), IHC, WB
Citations:
12
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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TUB-11, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

frog, mouse, chicken, human

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2-4 μg/mL using whole extract of cultured chicken fibroblasts

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBE1(51175)
mouse ... Tube1(71924)

General description

Tubulin epsilon 1 (ε-tubulin) is a member of tubulin superfamily. ε-tubulin is localized to the centrosome in cell-cycle-dependent manner. It is encoded by the gene mapped to human chromosome 6q21.
Monoclonal Anti-ε-Tubulin (mouse IgG2a isotype) is derived from the TUB-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide.

Immunogen

synthetic peptide corresponding to amino acids 352-366 of human ε-tubulin, conjugated to KLH.

Application

Monoclonal Anti-ε-Tubulin antibody produced in mouse may be used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunofluorescence

Biochem/physiol Actions

Monoclonal Anti-ε-Tubulin recognizes human, mouse, frog, and chicken ε-tubulin (55 kDa).
Tubulin ε 1 (ε-Tubulin) plays a vital role in microtubule anchoring and centriole maturation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Articles

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.


ε-Tubulin Is an Essential Component of the Centriole
Dutcher SK
Molecular Biology of the Cell, 13, 3859?3869-3859?3869 (2002)
Centrosome composition and microtubule anchoring mechanisms
Bornens M.
Current Opinion in Cell Biology, 14, 25-34 (2002)
S Doxsey
Nature reviews. Molecular cell biology, 2(9), 688-698 (2001-09-05)
Over the past 100 years, the centrosome has risen in status from an enigmatic organelle, located at the focus of microtubules, to a key player in cell-cycle progression and cellular control. A growing body of evidence indicates that centrosomes might



Global Trade Item Number

SKUGTIN
T1323-200UL04061838107633