T1788
Tyrode′s Solution, Acidic
liquid, sterile-filtered, suitable for mouse embryo cell culture
Synonym(s):
Cell culture solution
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Form:
liquid
Quality level:
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
pH
2.5±0.3
shipped in
dry ice
storage temp.
−20°C
General description
Tyrode′s salt solution is used for diluting protein components of media of natural origin. The current role of a balanced salt solution in cell culture is multifaceted. It serves as an irrigating, transporting, and diluting agent while maintaining intra- and extracellular osmotic balance and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It also provides the essential energy for cell metabolism and a buffering system to maintain the medium within the optimal pH range (7.2-7.6).
Application
Tyrode′s Solution, Acidic has been used for the removal of zona pellucida from oocytes/embryos.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
Zhao-Yang Xu et al.
Scientific reports, 5, 16978-16978 (2015-11-20)
Cep55 is a relatively novel member of the centrosomal protein family. Here, we show that Cep55 is expressed in mouse oocytes from the germinal vesicle (GV) to metaphase II (MII) stages. Immuostaining and confocal microscopy as well as time lapse
Sara Carvalhal et al.
Molecular biology of the cell, 28(19), 2470-2478 (2017-08-05)
Asymmetric cell divisions depend on the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center, but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small
Wenning Qin et al.
Genetics, 200(2), 423-430 (2015-03-31)
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is an adaptive immune system in bacteria and archaea that has recently been exploited for genome engineering. Mutant mice can be generated in one step through direct delivery of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T1788-100ML | 04061837338236 |