T1952
Trichostatin A, Ready Made Solution
5 mM in DMSO, from Streptomyces sp.
Synonym(s):
TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
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About This Item
Empirical Formula (Hill Notation):
C17H22N2O3
CAS Number:
Molecular Weight:
302.37
UNSPSC Code:
12352202
NACRES:
NA.32
Form:
DMSO solution
Assay:
≥98% (HPLC)
Biological source:
Streptomyces sp.
Quality Level
biological source
Streptomyces sp.
assay
≥98% (HPLC)
form
DMSO solution
concentration
5 mM in DMSO
technique(s)
cell culture | mammalian: suitable
antibiotic activity spectrum
fungi, neoplastics
mode of action
enzyme | inhibits
shipped in
dry ice
storage temp.
−20°C
SMILES string
N(O)C(=O)\C=C\C(=C\C(C)C(=O)c1ccc(cc1)N(C)C)\C
InChI
1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
InChI key
RTKIYFITIVXBLE-WKWSCTOISA-N
General description
Trichostatin A is a compound of primary hydroxamic acid.
Application
Trichostatin A, ready-made solution has been used:
- as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing
- to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)
- to treat cells for the HDAC inhibition experiments
Biochem/physiol Actions
Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.
Trichostatin A is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Bioprobes: Biochemical Tools for Investigating Cell Function, 11, 2104-2104 (2012)
Shujun Cai et al.
Molecular biology of the cell, 29(13), 1652-1663 (2018-05-10)
The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms
Valentina Saccone et al.
Genes & development, 28(8), 841-857 (2014-04-01)
Fibro-adipogenic progenitors (FAPs) are important components of the skeletal muscle regenerative environment. Whether FAPs support muscle regeneration or promote fibro-adipogenic degeneration is emerging as a key determinant in the pathogenesis of muscular diseases, including Duchenne muscular dystrophy (DMD). However, the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T1952-200UL | 04061837338465 |