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Merck
CN

T7791

Thymidine 5′-triphosphate sodium salt solution

10 mM (pH 7.0)

Synonym(s):

TTP

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About This Item

CAS Number:
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
41106305
MDL number:
Assay:
≥99%
Form:
liquid
Storage temp.:
−20°C
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assay

≥99%

Quality Level

form

liquid

feature

PCR Additives

concentration

10 mM (pH 7.0)

technique(s)

PCR: suitable

color

colorless

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

InChI key

KAELIXTVSANDSE-UHFFFAOYSA-N

General description

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

Application

Thymidine 5′-triphosphate sodium salt solution has been used:

  • as a standard to quantify each dNTP from cellular lysates by the liquid chromatography-mass spectrometry (LC-MS/MS) method
  • as a component of the dNTP mix for labeling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe
  • for routine PCR amplifications


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.





Global Trade Item Number

SKUGTIN
T7791-.5ML04061838218292