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Merck
CN

TOX8

In Vitro Toxicology Assay Kit, Resazurin based

Synonym(s):

Fluorescent resazurin toxicology assay

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About This Item

UNSPSC Code:
12352207
EC Number:
263-718-5
NACRES:
NA.84
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form

liquid

Quality Level

usage

1 kit sufficient for 2,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

protein quantification: suitable

application(s)

cell analysis
detection

detection method

colorimetric, fluorometric

storage temp.

2-8°C

General description

In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible

Application

In Vitro Toxicology Assay Kit, Resazurin based has been used:
  • in cell viability assay of human primary fibroblasts
  • to determine the cell proliferation rate in murine pre-osteoblasts
  • to determine the metabolic activity of microfluidic and monolayer cultures

Biochem/physiol Actions

Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).


Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

基于细胞的细胞增殖(BrdU、MTT、WST1)、细胞活力和细胞毒性实验,可应用于癌症、神经科学和干细胞研究。

用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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A comparative study of methods for assessing the toxicity to bacteria of single chemicals and mixtures.
King, E.F. et al.
Toxicity Screening Procedures Using Bacterial Systems, 175-194 (1984)
Ciarra Almeria et al.
Frontiers in bioengineering and biotechnology, 7, 292-292 (2019-11-12)
Mesenchymal stem/stromal cells (MSCs) display a variety of therapeutically relevant effects, such as the induction of angiogenesis, particularly under hypoxic conditions. It is generally recognized that MSCs exert their effects by secretion of paracrine factors and by stimulation of host
Maria Elisa Giuliani et al.
Toxicology in vitro : an international journal published in association with BIBRA, 61, 104603-104603 (2019-07-23)
The precision-cut tissue slices (PCTS) represent a largely used biological model in mammalian research. This ex vivo approach offers the main advantages of in vitro systems, while maintaining the natural architecture of the tissue. The use of PCTS in toxicological



Global Trade Item Number

SKUGTIN
TOX8-1KT04061837397974