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Y0875

Select Yeast Extract

for use in microbial growth medium

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About This Item

CAS Number:
UNSPSC Code:
41106212
NACRES:
NA.85
MDL number:
eCl@ss:
42040000
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grade

Molecular Biology

Quality Level

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

6.5-7.5(10% solution)

solubility

H2O: 100 mg/mL, clear to slightly hazy, yellow to brownish-yellow

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

SMILES string

OC(=O)CC1c2c3c(ccc2c4c1cccc4)cccc3

InChI

1S/C19H14O2/c20-18(21)11-17-15-8-4-3-7-14(15)16-10-9-12-5-1-2-6-13(12)19(16)17/h1-10,17H,11H2,(H,20,21)

InChI key

GQNBDGXKDJSVGQ-UHFFFAOYSA-N

General description

Yeast extract is the water-soluble portion of autolyzed yeast, often used in culture media. Yeast extract contains a mixture of amino acids, peptides, water soluble vitamins and carbohydrates.
Yeast extract is used in all solid media, on which stock cultures are maintained, due to its ability in promoting growth of most bacterial contaminants, thereby making them visible to identify. Yeast extract does not enhance the growth of wild-type cells but essential for auxotrophic mutants.

Application

Select Yeast Extract has been used:
  • as a supplement in the minimal sporulating medium (MSL) for the secretory production of human tumor necrosis factor-α (TNFα) using the carboxypeptidase Y (Cpy) signal peptide.
  • in the isolation of halophilic archaea.
  • as a component of microbial growth medium for cultivation of marine bacterial strain Zobellia uliginosa.

    Suitable for use as nutritional source in microbial media.
For general bacteriological use with a variety of microorganisms.
Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

Other Notes

Water soluble portion of autolyzed yeast with intact B-complex vitamins. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物源性产品

This item has



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Protocols

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

General protocols for growth of competent cells in microbial medium.


Chlamydomonas in the Laboratory
The Chlamydomonas Sourcebook (2009)
Søren Kjaerulff et al.
Biochemical and biophysical research communications, 336(3), 974-982 (2005-09-15)
In the fission yeast Schizosaccharomyces pombe, there are relatively few signal peptides available and most reports of their activity have not been comparative. Using sequence information from the S. pombe genome database we have identified three putative signal peptides, designated
Comparison of different signal peptides for secretion of heterologous proteins in fission yeast
Kjaerulff S and Jensen MR
Biochemical and Biophysical Research Communications, 336(3), 974-982 (2005)



Global Trade Item Number

SKUGTIN
Y0875-1KG04061826764985
Y0875-250G04061833655627