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Y2146

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

Synonym(s):

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.85
MDL number:
MFCD01095679
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Quality Level

200

form

powder

technique(s)

transformation: suitable

application(s)

microbiology

storage temp.

room temp

Application

Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Preparation Note

Dissolve 1.46 g of supplement per liter of medium.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
079M4161V
11190161
116M4081V
11161101
066M4054V

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Protocols

酵母生长实验方案

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast Drop Out

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Related Content

Data Sheet - Y2146

Instructions

Sudeshna Nag et al.
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
Riddhi Atul Jani et al.
Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.

Global Trade Item Number

SKUGTIN
Y2146-20G04061837588013