Y2377
2x YT medium
Powder microbial growth medium
Synonym(s):
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106225
grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
pH
6.8-7.2(3.1% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.
Features and Benefits
2xYT powder provides:
- Standard formulation
- An inexpensive alternative to liquid
Preparation Note
1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl
10 g/L Yeast Extract
5 g/L NaCl
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
监管及禁止进口产品
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Protocols
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
Related Content
Bacterial artificial chromosomes: A functional genomics tool for the study of positive-strand RNA viruses
Yun SI, et al.
Journal of Visualized Experiments, 106, e53164-e53164 (2015)
Telomere-mediated Chromosomal Truncation via Agrobacterium tumefaciens or Particle Bombardment to Produce Engineered Minichromosomes in Plants
Graham ND, et al.
Bio-protocol, 5(18) (2015)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Y2377-250G | 04061837588020 |