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A8094

L-Arginine

Name: <SC>L</SC>-Arginine
Brand: SIGMA

98.5-101.0%, suitable for cell culture, BioXtra, non-animal source

Synonym(s):

(S)-2-Amino-5-guanidinopentanoic acid

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About This Item

Linear Formula:

H₂NC(=NH)NH(CH₂)₃CH(NH₂)CO₂H

CAS Number:

74-79-3

Molecular Weight:

174.20

NACRES:

NA.26

PubChem Substance ID:

24891316

eCl@ss:

32160406

UNSPSC Code:

12352209

EC Number:

200-811-1

MDL number:

MFCD00002635

Beilstein/REAXYS Number:

1725413

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Properties

Product Name

L-Arginine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

biological source

non-animal source

Quality Level

300

agency

USP/NF, meets EP testing specifications, meets USP testing specifications

product line

BioXtra

assay

98.5-101.0%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

222 °C (dec.) (lit.)

solubility

H₂O: 100 mg/mL

application(s)

agriculture
peptide synthesis
pharmaceutical (small molecule)

SMILES string

N[C@@H](CCCNC(N)=N)C(O)=O

InChI

1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m0/s1

InChI key

ODKSFYDXXFIFQN-BYPYZUCNSA-N

Gene Information

human ... NOS1(4842), NOS2(4843)
rat ... Ppm1a(24666)

Description

Application

L-Arginine has been used:

as a Roswell park memorial institute medium (RPMI) media component in the isolation and culture of peripheral blood mononuclear cells (PBMCs)
as a RPMI media component for tissue culture
in DMEM medium for the identification and quantification of phosphorylation sites by stable isotope labeling by amino acids in cell culture (SILAC) and LCMS/MS

Biochem/physiol Actions

L-Arginine is a conditionally essential amino acid, that play a vital role in the production of nitric oxide. It acts as a precursor for the synthesis of polyamines, agmatine and urea. L-Arginine is usually essential in children and non-essential in adults.

Substrate of nitric oxide synthase, which is converted to citrulline and nitric oxide (NO). Induces insulin release by a nitric oxide-dependent mechanism.

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

四唑盐法(MTT)细胞活力和增殖检测方案

检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Global Trade Item Number

SKU	GTIN
A8094-10MG	04061833391181
A8094-1KG	04061833391198
A8094-25G	04061835521555
A8094-100G	04061833391174