A8781
β-Amylase from sweet potato
Synonym(s):
1,4-α-D-Glucan maltohydrolase
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About This Item
CAS Number:
UNSPSC Code:
41106514
NACRES:
NA.56
EC Number:
232-566-1
MDL number:
form
powder
Quality Level
mol wt
~200,000
packaging
vial of 15 mg
greener alternative product score
old score: 57
new score: 1
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greener alternative product characteristics
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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sustainability
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greener alternative category
storage temp.
−20°C
General description
β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.
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Application
β-Amylase from sweet potato has been used:
- as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)
- as a standard for calibration curve preparation in size exclusion chromatography
- as a model protein for small-angle X-ray analysis (SAXs)
Biochem/physiol Actions
β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Regulatory Information
低风险生物材料
常规特殊物品
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Protocols
Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.
Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand
Taniguchi, M. et al.
Journal of Fermentation and Bioengineering, 68, 32-36 (1989)
Liyu Tu et al.
Journal of cell science, 119(Pt 24), 5077-5086 (2006-12-13)
The surface of the mammalian urinary bladder is covered by a crystalline, asymmetric unit membrane (AUM) structure that contains the four major uroplakins (UPs): Ia, Ib, II and IIIa. UPIa and UPIb belong to the family of tetraspanins. Although UPIa
Integration of affinity precipitation with partitioning methods for bioseparation of chitin binding lectins.
Teotia, S. et al.
Food and Bioproducts Processing, 84, 37-43 (2006)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A8781-1VL | 04061838212160 |
| A8781-5X1VL | 04061833293225 |