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A9647

Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA

heat shock fraction, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥98%

Form:

lyophilized powder

Technique(s):

immunocytochemistry: suitable

Impurities:

≤5.0% Loss on drying

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Properties

biological source

bovine

assay

≥98%

form

lyophilized powder

purified by

heat shock fractionation

origin

USA origin

technique(s)

immunocytochemistry: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT virus, none detected, VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine serum albumin is a protein whose key biological function is to regulate the colloidal osmotic pressure of blood. BSA is widely used in various studies and applications. Made up of 583 amino acids, BSA is a water-soluble protein with a calculated molecular weight of 66,430 Da. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

Bovine Serum Albumin is used for many applications, including the following applications:

BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.
BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.
BSA has been used as a standard in the Bradford method of protein quantification.
BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number

0000279981

MJR0000002

SLCL4075

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Global Trade Item Number

SKU	GTIN
A9647-10G	04061835553716
A9647-500G	04061835553730
A9647-100G	04061835517657
A9647-1KG	04061835553723
A9647-50G	04061835511785
A9647-5G	04061833426005