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A9972

α-Amylase from human pancreas

Name: α-Amylase from human pancreas
Brand: SIGMA

lyophilized powder, ≥100 units/mg protein

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About This Item

CAS Number:

9000-90-2

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-565-6

NACRES:

NA.54

MDL number:

MFCD00081319

EC Number:

3.2.1.1 (BRENDA, IUBMB)

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Properties

biological source

human pancreas

form

lyophilized powder

specific activity

≥100 units/mg protein

purified by

2× crystallization

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

UniProt accession no.

P04746, P19961

greener alternative category

, Enabling

storage temp.

−20°C

Gene Information

human ... AMY1A(279), AMY2B(280)

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Biochem/physiol Actions

α-Amylase is composed of 496 amino acids in a single polypeptide chain. It hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Physical form

Lyophilized from Tris buffer containing NaCl and CaCl₂.

Preparation Note

Prepared by modified method of Levitzki et al.

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P342 + P311

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

高风险级别生物产品--人源产品

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Protocols

α-淀粉酶酶促测定 (EC 3.2.1.1)

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.