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Merck
CN

B0174

AccuTaq LA 10× Buffer

10X Buffer for long and accurate PCR

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About This Item

UNSPSC Code:
41106306
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grade

Molecular Biology

Quality Level

form

liquid

packaging

vial of 0.5 mL

concentration

10 ×

technique(s)

PCR: suitable

storage temp.

−20°C

General description

AccuTaq LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

Application

AccuTaq LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)

Legal Information

AccuTaq is a trademark of Sigma-Aldrich Co. LLC


hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品

This item has



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Protocols

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.


Baozhong Meng et al.
The Journal of general virology, 86(Pt 5), 1555-1560 (2005-04-16)
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA
Vandana Jaiswal et al.
PloS one, 10(6), e0129400-e0129400 (2015-06-16)
TaGW2 is an orthologue of rice gene OsGW2, which encodes E3 RING ubiquitin ligase and controls the grain size in rice. In wheat, three copies of TaGW2 have been identified and mapped on wheat homoeologous group 6 viz. TaGW2-6A, TaGW2-6B



Global Trade Item Number

SKUGTIN
B0174-.5ML04061838121363
B0174-5ML04061837031991