B9643
Bicinchoninic Acid solution
Synonym(s):
BCA
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352106
Form:
liquid
General description
Protein determination is one of the most common operations performed in biochemical research. Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. Both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. The BCA assay is more sensitive and applicable than either biuret or Lowry procedures.
Application
Bicinchoninic Acid solution (Bicinchoninic acid protein assay kit) is used :
- For the determination of total protein content in the process of quantifying bromelain, extracted from the pineapple stem residues using aqueous micellar two-phase systems composed of ionic liquids as co-surfactants.
- For the determination of bovine serum albumin (BSA) in biomimetic calcium phosphate coating co-precipitated on titanium alloy plates.
This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.
Features and Benefits
- It is easy to use.
- The color complex is stable.
- There is less susceptibility to detergents.
- It is applicable over a broad range of protein concentrations.
Packaging
Also available as part of the Bicinchoninic Acid Kit for Protein Determination (BCA-1).
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
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Protocols
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Related Content
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Diego García-Ayuso et al.
Experimental eye research, 161, 10-16 (2017-05-30)
In this work we study the effects of an acute light-induced retinal degeneration on the population of melanopsin positive retinal ganglion cells (m
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente AF, et al.
Process Biochemistry (Oxford, United Kingdom), 51(4), 528-534 (2016)
Shabnam Khatibi et al.
BMC systems biology, 11(1), 48-48 (2017-04-15)
Transforming growth factor β (TGF-β) signalling regulates the development of embryos and tissue homeostasis in adults. In conjunction with other oncogenic changes, long-term perturbation of TGF-β signalling is associated with cancer metastasis. Although TGF-β signalling can be complex, many of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B9643-1L | 04061838251657 |