C0615
Cellulase from Trichoderma sp.
powder, ≥5,000 units/g solid
Synonym(s):
Onozuka RS
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
eCl@ss:
32160410
Specific activity:
≥5,000 units/g solid
form
powder
Quality Level
specific activity
≥5,000 units/g solid
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
solubility
deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB))
greener alternative category
storage temp.
2-8°C
General description
Manufactured by Yakult
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research
Application
Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties. Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose while investigating the biodegradability of bioabsorbable bacterial cellulose (BBC).
Biochem/physiol Actions
Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences .
Preparation Note
Dissolves in sterile deionized (DI) water in the presence of 0.15 polyhexamethylene biguanide (PHMB) at 3 mg/mL concentration.
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Regulatory Information
常规特殊物品
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Protocols
该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。
To standardize an enzymatic assay procedure of cellulase.
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Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0615-1G | 04061835555765 |