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C9584

Carboxypeptidase B from porcine pancreas

lyophilized powder

Synonym(s):

Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase

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About This Item

CAS Number:
9025-24-5
PubChem Substance ID:
24893175
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-788-9
MDL number:
MFCD00081476
EC Number:
3.4.17.2 (BRENDA, IUBMB)
Specific activity:
≥125 units/mg protein
Biological source:
Porcine pancreas
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biological source

Porcine pancreas

Quality Level

200

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥125 units/mg protein

mol wt

34,000 Da± 600

purified by

affinity chromatography

composition

Protein, 40-70%

foreign activity

carboxypeptidase A ≤1%, chymotrypsin ≤0.1%, trypsin ≤5%

storage temp.

−20°C

SMILES string

S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1

InChI

1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)

InChI key

TWURVFFNODFJBJ-UHFFFAOYSA-N

Application

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Biochem/physiol Actions

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.

Packaging

Package size based on protein content

Physical form

Contains HEPES buffer salts, zinc chloride and carbohydrate

Preparation Note

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

wgk

WGK 3

ppe

Eyeshields, Gloves

Storage Class

11 - Combustible Solids

flash_point_f

No data available

flash_point_c

No data available

Regulatory Information

低风险生物材料
动植物源性产品

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL3134
SLCD6680
SLCB2773
SLBX8638
SLBR9625V

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Related Content

Laboratory diagnosis of acute pancreatitis: in search of the Holy Grail.
Lippi, G. et al.
Clinical Laboratory Science : Journal of the American Society For Medical Technology, 49, 18-31 (2012)
Marco Candela et al.
Journal of bacteriology, 189(16), 5929-5936 (2007-06-15)
Bifidobacteria constitute up to 3% of the total microbiota and represent one of the most important health-promoting bacterial groups of the human intestinal microflora. The presence of Bifidobacterium in the human gastrointestinal tract has been directly related to several health-promoting
Marc Ferrer et al.
Analytical biochemistry, 317(1), 94-98 (2003-05-06)
An epitope-unmasking, homogeneous time-resolved fluorescence (HTRF) assay has been developed for measuring carboxypeptidase B (CPB) activity in a miniaturized high-throughput screening format. The enzyme substrate (biotin-RYRGLMVGGVVR-OH) is cleaved by CPB at the C terminus, causing release of the C-terminal Arg
View All Related Papers

Global Trade Item Number

SKUGTIN
C9584-1MG04061833530894
C9584-5MG04061833530900