Key Documents

View All Documentation

HeLa Cell Line human

Name: HeLa Cell Line human
Brand: SIGMA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93021013, human cervix, Epithelial

Synonym(s):

HeLa Cells, HeLa-CCL2 Cells, Henrietta Lacks Cells

Select a Size

Change View

About This Item

UNSPSC Code:

41106514

Biological source:

human cervix

Relevant disease(s):

cancer

Growth mode:

Adherent

Karyotype:

aneuploidy

Morphology:

Epithelial

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

human cervix

packaging

tube of 5 μg 93021013-DNA-5UG, pkg of vial of cells 93021013-1VL

growth mode

Adherent

karyotype

aneuploidy

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Description

Application

HeLa cell line has been used to study whether Zika virus (ZIKV) infection alters centrosome number and spindle positioning in neural progenitor cells. It has also been used to evaluate the cytotoxicity of extracts, fruits and jams from Sorbus sps.

Virus studies

Biochem/physiol Actions

Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Human cervix epitheloid carcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 10% Foetal Bovine Serum FBS / FCS.

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm² using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

低风险生物材料

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Evaluation of Spheroid Formation and Robustness using Millicell^® Ultra-low Attachment Plates

Learn how to quantify spheroid formation, roundness, and circularity. Discover Millicell^® Ultra-low Attachment plates, an ideal 3D cell culture model for uniform spheroid formation.

Millicell^® Ultra-Low Attachment Plates Spheroid Formation Performance Comparison

Learn about the similarities and differences in spheroid formation, roundness, and circularity between Millicell^{®^{ultra-low attachment plates and other ULA plates.}}

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.