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Merck
CN

D3937

DirectLoad 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

1 kb marker, 1kb gel marker, DNA marker, agarose gel electrophoresis marker

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105335
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Quality Level

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

Application

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Other Notes

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s DirectLoad 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC


Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

This item has



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Protocols

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

View All Protocols

Articles

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Related Content


Aron M Geurts et al.
Methods in molecular biology (Clifton, N.J.), 597, 211-225 (2009-12-17)
The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat



Global Trade Item Number

SKUGTIN
D3937-1VL04061835572588