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D8537

Dulbecco’s Phosphate Buffered Saline

Name: Dulbecco’s Phosphate Buffered Saline
Brand: SIGMA

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

UNSPSC Code:

12352207

eCl@ss:

32129211

NACRES:

NA.75

MDL number:

MFCD00131855

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Properties

Product Name

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

storage temp.

room temp

Description

General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.
to wash the wells of a microtiter plate.
to wash the cells before resuspending them in lysis buffer for DNA isolation.
as a component of embryo holding medium for vitrification of embryos.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
D8537-6X500ML	04061838259400
D8537-100ML	04061833589724
D8537-1L	04061838259370
D8537-24X500ML	04061833589731
D8537-500ML	04061838259387
D8537-6X1L	04061838259394