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E2264

Endoglycosidase F3 from Elizabethkingia miricola

Name: Endoglycosidase F3 from <I>Elizabethkingia miricola</I>
Brand: SIGMA

recombinant, expressed in E. coli, 30 U/mg

Synonym(s):

Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.2.1.96 (BRENDA, IUBMB)

MDL number:

MFCD00146843

Specific activity:

30 U/mg

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Level

200

conjugate

(N-linked)

form

solution

specific activity

30 U/mg

mol wt

32 kDa

shipped in

wet ice

storage temp.

2-8°C

Description

Application

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

Biochem/physiol Actions

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Packaging

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Physical form

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Articles

内切糖苷酶

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Global Trade Item Number

SKU	GTIN
E2264-.2UN	04061833602560